LONG-TERM FROZEN STORAGE OF MAMMALIAN CELL LINES
نویسندگان
چکیده
منابع مشابه
Isolation and long-term culture of diploid mammalian cell lines.
Cells have been established in continuous culture from human and animal tissues, both normal and carcinomatous. From 197 specimens, 105 could be maintained in vitro for varying lengths of time, 27 for more than 6 months. In all cases the cells were found to be diploid. Cell lines with both epithelial and fibroblastic morphology have been isolated in medium 199 with added fetal calf serum, but a...
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Background: The aim of this study was to develop methods for cryopreservation and long-term maintenance of putative bovine embryonic stem cells (ESCs). Materials and Methods: Putative bovine ESC (bESC) lines (n=3) isolated in conventional medium were used to compare slow-freezing and vitrification. Results: After warming, vitrified cells (96.9%) demonstrated significantly (p<0.05) better surviv...
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This study examined the effects of long-term frozen storage (-20 °C for 5 months) of free amino acids (FAAs) and nucleotide-related compounds (NRCs) in muscle and hepatopancreas of the coconut crab Birgus latro. Although long-term frozen storage had little effect on FAA composition in muscle, the amounts of several FAAs increased in the hepatopancreas that may be the result of protein decom...
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متن کاملEffects of long-term frozen storage on the compositions of free amino acids and nucleotide-related compounds of the coconut crab Birgus latro
This study examined the effects of long-term frozen storage (-20 °C for 5 months) of free amino acids (FAAs) and nucleotide-related compounds (NRCs) in muscle and hepatopancreas of the coconut crab Birgus latro. Although long-term frozen storage had little effect on FAA composition in muscle, the amounts of several FAAs increased in the hepatopancreas that may be the result of protein decomposi...
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ژورنال
عنوان ژورنال: Japanese Journal of Medical Science and Biology
سال: 1961
ISSN: 0021-5112,1884-2828
DOI: 10.7883/yoken1952.14.27